Open Access

Proteomic profiling of human HepG2 cells treated with hesperidin using antibody array

  • Authors:
    • Silvia Yumnam
    • Venu Venkatarame Gowda Saralamma
    • Suchismita Raha
    • Ho Jeong Lee
    • Won Sup Lee
    • Eun Kim Kim
    • Sang Joon Lee
    • Jeong Doo Heo
    • Gon Sup Kim
  • View Affiliations

  • Published online on: August 11, 2017     https://doi.org/10.3892/mmr.2017.7232
  • Pages: 5386-5392
  • Copyright: © Yumnam et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Protein array technology not only identifies a large number of proteins but also determines their expression levels. In the present study, antibody array analysis is used to decipher the proteins involved in hesperidin-induced cell death in HepG2 cells. Altered proteins in hesperidin treated cells were compared with that of untreated control cells by using a RayBio® Label‑based (L series) human antibody array kit. The identified proteins were further confirmed using western blot analysis. STRING software based analysis was used to determine the protein‑protein interactions. Many proteins related to signal transduction, cellular mechanisms, cell growth and proliferation regulatory proteins were identified. Among the proteins identified Hsp90, Smac/DIABLO, Prdx6 and FRK were significantly reduced in hesperidin treated cells. To the best of the authors' knowledge, the present study is the first to use antibody array for identifying proteins marker in hesperidin‑induced cell death in HepG2 cells. The present study provides a novel insight into the anticancer mechanism of hesperidin.

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October-2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Yumnam S, Venkatarame Gowda Saralamma V, Raha S, Lee HJ, Lee WS, Kim EK, Lee SJ, Heo JD and Kim GS: Proteomic profiling of human HepG2 cells treated with hesperidin using antibody array. Mol Med Rep 16: 5386-5392, 2017.
APA
Yumnam, S., Venkatarame Gowda Saralamma, V., Raha, S., Lee, H.J., Lee, W.S., Kim, E.K. ... Kim, G.S. (2017). Proteomic profiling of human HepG2 cells treated with hesperidin using antibody array. Molecular Medicine Reports, 16, 5386-5392. https://doi.org/10.3892/mmr.2017.7232
MLA
Yumnam, S., Venkatarame Gowda Saralamma, V., Raha, S., Lee, H. J., Lee, W. S., Kim, E. K., Lee, S. J., Heo, J. D., Kim, G. S."Proteomic profiling of human HepG2 cells treated with hesperidin using antibody array". Molecular Medicine Reports 16.4 (2017): 5386-5392.
Chicago
Yumnam, S., Venkatarame Gowda Saralamma, V., Raha, S., Lee, H. J., Lee, W. S., Kim, E. K., Lee, S. J., Heo, J. D., Kim, G. S."Proteomic profiling of human HepG2 cells treated with hesperidin using antibody array". Molecular Medicine Reports 16, no. 4 (2017): 5386-5392. https://doi.org/10.3892/mmr.2017.7232