MicroRNA-181a sensitizes human malignant glioma U87MG cells to radiation by targeting Bcl-2

  • Authors:
    • Gong Chen
    • Wei Zhu
    • Dezhi Shi
    • Li Lv
    • Chun Zhang
    • Ping Liu
    • Weixing Hu
  • View Affiliations

  • Published online on: April 1, 2010     https://doi.org/10.3892/or_00000725
  • Pages: 997-1003
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Abstract

Radiotherapy is widely used in cancer treatment and biological studies. Multiple mechanisms induced by radiation, especially changes of the expression profile of genes, lead to the disruption of cellular homeostasis. MicroRNAs (miRNAs) are important post-transcriptional gene regulators and play an important role in response to cellular stress. Here we investigated the profiles of miRNA expression following exposure to radiation and the possible role of miRNAs in the modulation of radiosensitivity in the glioblastoma multiform U87MG cell line. MiRNA expression profiles revealed a limited set of miRNAs with altered expression in U87MG cells in response to radiation treatment. MiR-181a, a member of miR-181 family, was one of the down-regulated miRNAs, whose expression was further validated by qRT-PCR. The target mRNAs of radiation-responsive miRNAs were predicted with a target prediction tool. Transiently overexpressed miR-181a significantly sensitized malignant glioma cells to radiation treatment concurrent with the down-regulation of the protein Bcl-2 (B cell lymphoma/lewkmia-2). It indicates that miR-181a may modulate radiosensitivity by targeting Bcl-2 in human malignant glioma cells. These data suggest that radiation can affect miRNA expression, which regulates the cellular response, and miR-181a could be a target for enhancing the effect of radiation treatment on malignant glioma cells.

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April 2010
Volume 23 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chen G, Zhu W, Shi D, Lv L, Zhang C, Liu P and Hu W: MicroRNA-181a sensitizes human malignant glioma U87MG cells to radiation by targeting Bcl-2 . Oncol Rep 23: 997-1003, 2010.
APA
Chen, G., Zhu, W., Shi, D., Lv, L., Zhang, C., Liu, P., & Hu, W. (2010). MicroRNA-181a sensitizes human malignant glioma U87MG cells to radiation by targeting Bcl-2 . Oncology Reports, 23, 997-1003. https://doi.org/10.3892/or_00000725
MLA
Chen, G., Zhu, W., Shi, D., Lv, L., Zhang, C., Liu, P., Hu, W."MicroRNA-181a sensitizes human malignant glioma U87MG cells to radiation by targeting Bcl-2 ". Oncology Reports 23.4 (2010): 997-1003.
Chicago
Chen, G., Zhu, W., Shi, D., Lv, L., Zhang, C., Liu, P., Hu, W."MicroRNA-181a sensitizes human malignant glioma U87MG cells to radiation by targeting Bcl-2 ". Oncology Reports 23, no. 4 (2010): 997-1003. https://doi.org/10.3892/or_00000725