miR-497 induces apoptosis of breast cancer cells by targeting Bcl-w

  • Authors:
    • Lei Shen
    • Jia Li
    • Liping Xu
    • Jie Ma
    • Hua Li
    • Xigang Xiao
    • Junyong Zhao
    • Lin Fang
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  • Published online on: December 22, 2011     https://doi.org/10.3892/etm.2011.428
  • Pages: 475-480
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Abstract

microRNAs are a small class of non-coding RNAs with a critical role in the tumorigenesis and maintenance of breast cancer through binding to the 3'‑untranslated regions of target mRNAs, which causes a block of translation and/or mRNA degradation. The purpose of this study was to investigate the expression of microRNA-497 (miR-497) as well as its potential role in human breast cancer. Reverse transcription-polymerase chain reaction (RT-PCR) was performed to determine the expression pattern of miR-497 in breast cancer and normal breast tissues. Correlation analysis was conducted to characterize the association of miR-497 expression abnormality with pathological factors. Proliferation, cell cycle and apoptosis assays were conducted to explore the potential function of miR-497 in human MCF-7 breast cancer cells. RT-PCR and Western blot analysis were employed to validate the downstream targets of miR-497. miR-497 expression was relatively decreased in breast cancer specimens and negatively correlated with TNM stage, lymphatic metastasis, tumor size and human epidermal growth factor receptor-2 (P<0.01). On the contrary, no correlation was found with estrogen receptor, progesterone receptor and p53 status. Functional assays revealed that miR-497 suppressed cellular growth, increased the percentage of early apoptotic cells and initiated G0/G1 cell phase arrest of MCF-7 cancer cells. RT-PCR and Western blot analysis data indicated that the overexpression of miR-497 resulted in the down‑regulation of Bcl-w at the mRNA and protein levels. miR-497 may serve as a tumor suppressor gene in breast cancer. The Up-regulation of miR-497 expression causes cellular growth inhibition and apoptotic enhancement, as well as G0/G1 phase arrest, suggesting its use as a potential therapeutic target for the treatment of breast cancer in the future.
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March 2012
Volume 3 Issue 3

Print ISSN: 1792-0981
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Spandidos Publications style
Shen L, Li J, Xu L, Ma J, Li H, Xiao X, Zhao J and Fang L: miR-497 induces apoptosis of breast cancer cells by targeting Bcl-w. Exp Ther Med 3: 475-480, 2012.
APA
Shen, L., Li, J., Xu, L., Ma, J., Li, H., Xiao, X. ... Fang, L. (2012). miR-497 induces apoptosis of breast cancer cells by targeting Bcl-w. Experimental and Therapeutic Medicine, 3, 475-480. https://doi.org/10.3892/etm.2011.428
MLA
Shen, L., Li, J., Xu, L., Ma, J., Li, H., Xiao, X., Zhao, J., Fang, L."miR-497 induces apoptosis of breast cancer cells by targeting Bcl-w". Experimental and Therapeutic Medicine 3.3 (2012): 475-480.
Chicago
Shen, L., Li, J., Xu, L., Ma, J., Li, H., Xiao, X., Zhao, J., Fang, L."miR-497 induces apoptosis of breast cancer cells by targeting Bcl-w". Experimental and Therapeutic Medicine 3, no. 3 (2012): 475-480. https://doi.org/10.3892/etm.2011.428