Differential expression of plasma miR-146a in sepsis patients compared with non-sepsis-SIRS patients

  • Authors:
    • Lina Wang
    • Hua-Cheng Wang
    • Cha Chen
    • Jianming Zeng
    • Qian Wang
    • Lei Zheng
    • Huan-Du Yu
  • View Affiliations

  • Published online on: January 30, 2013     https://doi.org/10.3892/etm.2013.937
  • Pages: 1101-1104
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Abstract

Sepsis is a subtype of systemic inflammatory response syndrome (SIRS), which is characterized by infection. Circulating microRNAs (miRNAs), including miR-150, miR-146a and miR-223, are potential biomarkers of sepsis. In this study, we demonstrated that measuring the relative expression of miR-146a/U6 in plasma, using the 2-∆∆Ct method, provides a method for differentiating between sepsis and non-sepsis-SIRS. We observed a significant increase in miR-146a expression in the initial cohort of 6 non-sepsis-SIRS patients compared to the 4 sepsis patients (P=0.01) and in the second cohort of 8 non-sepsis-SIRS patients compared to the 10 sepsis patients (P=0.027). Additionally, we identified that sodium citrate and ethylenediaminetetraacetic acid (EDTA) K2 may be used as anticoagulant reagents. Generation of a standard curve is not necessary in these diagnostic tests, unless the standard of normalization is carefully selected. Thus we provide more detailed guidance for the clinical use of circulating miRNA biomarkers.
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April 2013
Volume 5 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang L, Wang H, Chen C, Zeng J, Wang Q, Zheng L and Yu H: Differential expression of plasma miR-146a in sepsis patients compared with non-sepsis-SIRS patients. Exp Ther Med 5: 1101-1104, 2013.
APA
Wang, L., Wang, H., Chen, C., Zeng, J., Wang, Q., Zheng, L., & Yu, H. (2013). Differential expression of plasma miR-146a in sepsis patients compared with non-sepsis-SIRS patients. Experimental and Therapeutic Medicine, 5, 1101-1104. https://doi.org/10.3892/etm.2013.937
MLA
Wang, L., Wang, H., Chen, C., Zeng, J., Wang, Q., Zheng, L., Yu, H."Differential expression of plasma miR-146a in sepsis patients compared with non-sepsis-SIRS patients". Experimental and Therapeutic Medicine 5.4 (2013): 1101-1104.
Chicago
Wang, L., Wang, H., Chen, C., Zeng, J., Wang, Q., Zheng, L., Yu, H."Differential expression of plasma miR-146a in sepsis patients compared with non-sepsis-SIRS patients". Experimental and Therapeutic Medicine 5, no. 4 (2013): 1101-1104. https://doi.org/10.3892/etm.2013.937