The anthelmintic agent oxfendazole inhibits cell growth in non‑small cell lung cancer by suppressing c‑Src activation

  • Authors:
    • Dafu Xu
    • Wenze Tian
    • Chao Jiang
    • Ziming Huang
    • Shiying Zheng
  • View Affiliations

  • Published online on: January 28, 2019     https://doi.org/10.3892/mmr.2019.9897
  • Pages: 2921-2926
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Abstract

The c‑Src protein family of tyrosine kinases are important in the tumorigenesis of many types of tumors, and may be a potential target for antitumor drug discovery. In the present study, immunoblotting was performed to analyze protein expression, CCK‑8 assay was carried out to assess cell viability and cell cycle was analyzed using a flow cytometer. The anthelmintic agent oxfendazole was observed to be a novel c‑Src inhibitor that blocked the activation of c‑Src. Oxfendazole also suppressed the cell growth of non‑small cell lung cancer (NSCLC) cells, and overexpression of c‑Src decreased the cytotoxicity of oxfendazole against NSCLC cells. In addition, oxfendazole induced cell cycle arrest at the G0/G1 phase, and downregulated the protein levels of Cyclin‑dependent kinase (CDK)‑4, CDK6, retinoblastoma protein and E2 transcription factor 1, and upregulated the expression levels of p53 and p21 in NSCLC cells. Furthermore, oxfendazole enhanced the cytotoxicity of cisplatin against NSCLC cells. These results demonstrated that oxfendazole exerted its antitumor activity by suppressing c‑Src signaling, and it was also indicated that the anthelmintic agent oxfendazole may be effective for anti‑NSCLC therapy in the clinic as a single agent or in combination with other antitumor drugs.
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April-2019
Volume 19 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Xu D, Tian W, Jiang C, Huang Z and Zheng S: The anthelmintic agent oxfendazole inhibits cell growth in non‑small cell lung cancer by suppressing c‑Src activation . Mol Med Rep 19: 2921-2926, 2019.
APA
Xu, D., Tian, W., Jiang, C., Huang, Z., & Zheng, S. (2019). The anthelmintic agent oxfendazole inhibits cell growth in non‑small cell lung cancer by suppressing c‑Src activation . Molecular Medicine Reports, 19, 2921-2926. https://doi.org/10.3892/mmr.2019.9897
MLA
Xu, D., Tian, W., Jiang, C., Huang, Z., Zheng, S."The anthelmintic agent oxfendazole inhibits cell growth in non‑small cell lung cancer by suppressing c‑Src activation ". Molecular Medicine Reports 19.4 (2019): 2921-2926.
Chicago
Xu, D., Tian, W., Jiang, C., Huang, Z., Zheng, S."The anthelmintic agent oxfendazole inhibits cell growth in non‑small cell lung cancer by suppressing c‑Src activation ". Molecular Medicine Reports 19, no. 4 (2019): 2921-2926. https://doi.org/10.3892/mmr.2019.9897