Caspase-14 is an anti-apoptotic protein targeting apoptosis-inducing factor in lung adenocarcinomas

  • Authors:
    • Hsin-Yuan Fang
    • Chih-Yi Chen
    • Min-Fa Hung
    • Yi-Ting Hsiao
    • Ting-Chieh Chiang
    • Tze-Yi Lin
    • Hui-Wen Chang
    • Kuan-Chih Chow
    • Wen-Je Ko
  • View Affiliations

  • Published online on: April 29, 2011     https://doi.org/10.3892/or.2011.1292
  • Pages: 359-369
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Abstract

Using apoptosis-inducing factor protein as bait in a yeast hybrid assay to screen protein libraries, we identified three proteins that interacted with apoptosis-inducing factor: human homolog of yeast Rad23 protein A (hHR23A), microsomal glutathione S-transferase and caspase-14 (casp-14). In this study, we investigated the expression and function of casp-14 in lung adenocarcinomas (LADC). Our results showed that monoclonal antibodies were specific to casp-14, and that casp-14 was highly expressed in LADC. Casp-14 overexpression correlated with tumor stage, cell differentiation and lymphovascular involvement, suggesting that casp-14 was associated with tumor cell growth and metastatic potential. In vitro, casp-14 interacted with apoptosis-inducing factor, and silencing of casp-14 expression reduced cisplatin resistance. Our data suggest that casp-14 is an anti-apoptotic protein targeting apoptosis-inducing factor and increases cisplatin resistance in LADC cells.

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August 2011
Volume 26 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Fang H, Chen C, Hung M, Hsiao Y, Chiang T, Lin T, Chang H, Chow K and Ko W: Caspase-14 is an anti-apoptotic protein targeting apoptosis-inducing factor in lung adenocarcinomas. Oncol Rep 26: 359-369, 2011.
APA
Fang, H., Chen, C., Hung, M., Hsiao, Y., Chiang, T., Lin, T. ... Ko, W. (2011). Caspase-14 is an anti-apoptotic protein targeting apoptosis-inducing factor in lung adenocarcinomas. Oncology Reports, 26, 359-369. https://doi.org/10.3892/or.2011.1292
MLA
Fang, H., Chen, C., Hung, M., Hsiao, Y., Chiang, T., Lin, T., Chang, H., Chow, K., Ko, W."Caspase-14 is an anti-apoptotic protein targeting apoptosis-inducing factor in lung adenocarcinomas". Oncology Reports 26.2 (2011): 359-369.
Chicago
Fang, H., Chen, C., Hung, M., Hsiao, Y., Chiang, T., Lin, T., Chang, H., Chow, K., Ko, W."Caspase-14 is an anti-apoptotic protein targeting apoptosis-inducing factor in lung adenocarcinomas". Oncology Reports 26, no. 2 (2011): 359-369. https://doi.org/10.3892/or.2011.1292