Antitumor effect of 1, 8-cineole against colon cancer

  • Authors:
    • Soichiro Murata
    • Risa Shiragami
    • Chihiro Kosugi
    • Tohru Tezuka
    • Masato Yamazaki
    • Atsushi Hirano
    • Yukino Yoshimura
    • Masato Suzuki
    • Kiyohiko Shuto
    • Nobuhiro Ohkohchi
    • Keiji Koda
  • View Affiliations

  • Published online on: October 1, 2013     https://doi.org/10.3892/or.2013.2763
  • Pages: 2647-2652
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Abstract

Several essential oils possess pharmacological effects. Among the various constituents of essential oils, 1, 8-cineole has been shown to possess pharmacological effects such as anti-bacterial and anti-inflammatory effects. The effect of 1, 8-cineole on human colorectal cancer cells, however, has not reported previously. In this study, we have investigated the anti-proliferative effect of 1, 8-cineole on human colon cancer cell lines HCT116 and RKO by WST-8 and BrdU assays. The cytotoxicity of 1, 8-cineole was investigated by LDH activity and TUNEL staining. The mechanism of apoptosis by 1, 8-cineole was determined by western blot analyses. In in vivo study, RKO cells were injected into the SCID mice and the effect of 1, 8-cineole was investigated. Specific induction of apoptosis, not necrosis, was observed in human colon cancer cell lines HCT116 and RKO by 1, 8-cineole. The treatment with 1, 8-cineole was associated with inactivation of survivin and Akt and activation of p38. These molecules induced cleaved PARP and caspase-3, finally causing apoptosis. In xenotransplanted SCID mice, the 1, 8-cineole group showed significantly inhibited tumor progression compared to the control group. These results indicated 1, 8-cineole suppressed human colorectal cancer proliferation by inducing apoptosis. Based on these studies 1, 8-cineole would be an effective strategy to treat colorectal cancer.
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December 2013
Volume 30 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Murata S, Shiragami R, Kosugi C, Tezuka T, Yamazaki M, Hirano A, Yoshimura Y, Suzuki M, Shuto K, Ohkohchi N, Ohkohchi N, et al: Antitumor effect of 1, 8-cineole against colon cancer. Oncol Rep 30: 2647-2652, 2013.
APA
Murata, S., Shiragami, R., Kosugi, C., Tezuka, T., Yamazaki, M., Hirano, A. ... Koda, K. (2013). Antitumor effect of 1, 8-cineole against colon cancer. Oncology Reports, 30, 2647-2652. https://doi.org/10.3892/or.2013.2763
MLA
Murata, S., Shiragami, R., Kosugi, C., Tezuka, T., Yamazaki, M., Hirano, A., Yoshimura, Y., Suzuki, M., Shuto, K., Ohkohchi, N., Koda, K."Antitumor effect of 1, 8-cineole against colon cancer". Oncology Reports 30.6 (2013): 2647-2652.
Chicago
Murata, S., Shiragami, R., Kosugi, C., Tezuka, T., Yamazaki, M., Hirano, A., Yoshimura, Y., Suzuki, M., Shuto, K., Ohkohchi, N., Koda, K."Antitumor effect of 1, 8-cineole against colon cancer". Oncology Reports 30, no. 6 (2013): 2647-2652. https://doi.org/10.3892/or.2013.2763