H19 lncRNA mediates 17β-estradiol-induced cell proliferation in MCF-7 breast cancer cells

  • Authors:
    • Hong Sun
    • Guo Wang
    • Yan Peng
    • Ying Zeng
    • Qiong-Ni Zhu
    • Tai-Lin Li
    • Jia-Qin Cai
    • Hong-Hao Zhou
    • Yuan-Shan Zhu
  • View Affiliations

  • Published online on: April 3, 2015     https://doi.org/10.3892/or.2015.3899
  • Pages: 3045-3052
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Estrogen plays a critical role in breast cancer development and progression. However, the mechanism involved in the promotion of breast cancer development and progression by estrogen remains unclear although it has been intensively studied. In the present study, we investigated the estrogen inducibility and functional significance of H19 lncRNA in breast cancer cells and tumor tissues. The screening of 83 disease-related long non-coding RNAs (lncRNAs) revealed that H19 lncRNA was much higher in estrogen receptor (ER)-positive MCF-7 breast cancer cells than in ER-negative MDA-MB-231 cells. 17β-estradiol produced a dose- and time-dependent induction of H19 expression in MCF-7 cells, which was mediated via ERα as evident by the blockade of this 17β-estradiol effect with ICI 182780, a specific ER antagonist and knockdown of ERα using specific RNAi. Moreover, knockdown of H19 lncRNA decreased cell survival and blocked estrogen-induced cell growth while overexpression of H19 lncRNA stimulated cell proliferation. Quantitation of H19 lncRNA in human breast cancer tissues showed that the level of H19 lncRNA was >10-fold higher in ER-positive than in ER-negative tumor tissues. These results suggest that H19 is an estrogen-inducible gene and plays a key role in cell survival and in estrogen-induced cell proliferation in MCF-7 cells, indicating that H19 lncRNA may serve as a biomarker for breast cancer diagnosis and progression, and as a valuable target for breast cancer therapy.
View Figures
View References

Related Articles

Journal Cover

June-2015
Volume 33 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Sun H, Wang G, Peng Y, Zeng Y, Zhu Q, Li T, Cai J, Zhou H and Zhu Y: H19 lncRNA mediates 17β-estradiol-induced cell proliferation in MCF-7 breast cancer cells. Oncol Rep 33: 3045-3052, 2015.
APA
Sun, H., Wang, G., Peng, Y., Zeng, Y., Zhu, Q., Li, T. ... Zhu, Y. (2015). H19 lncRNA mediates 17β-estradiol-induced cell proliferation in MCF-7 breast cancer cells. Oncology Reports, 33, 3045-3052. https://doi.org/10.3892/or.2015.3899
MLA
Sun, H., Wang, G., Peng, Y., Zeng, Y., Zhu, Q., Li, T., Cai, J., Zhou, H., Zhu, Y."H19 lncRNA mediates 17β-estradiol-induced cell proliferation in MCF-7 breast cancer cells". Oncology Reports 33.6 (2015): 3045-3052.
Chicago
Sun, H., Wang, G., Peng, Y., Zeng, Y., Zhu, Q., Li, T., Cai, J., Zhou, H., Zhu, Y."H19 lncRNA mediates 17β-estradiol-induced cell proliferation in MCF-7 breast cancer cells". Oncology Reports 33, no. 6 (2015): 3045-3052. https://doi.org/10.3892/or.2015.3899