Open Access

Inhibitor of Wnt receptor 1 suppresses the effects of Wnt1, Wnt3a and β‑catenin on the proliferation and migration of C6 GSCs induced by low‑dose radiation

  • Authors:
    • Yu Yan
    • Ying-Ying Cheng
    • Yan-Ru Li
    • Xu-Wen Jiao
    • Yin-Ming Liu
    • Hai-Yan Cai
    • Yin-Xiu Ding
  • View Affiliations

  • Published online on: December 12, 2023     https://doi.org/10.3892/or.2023.8681
  • Article Number: 22
  • Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The radioresistance of glioma is an important cause of treatment failure and tumor aggressiveness. In the present study, under performed with linear accelerator, the effects of 0.3 and 3.0 Gy low‑dose radiation (LDR) on the proliferation and migration of C6 glioma stem cells in vitro were examined by flow cytometric analysis, immunocytochemistry and western blot analysis. It was found that low‑dose ionizing radiation (0.3 Gy) stimulated the proliferation and migration of these cells, while 3.0 Gy ionizing radiation inhibited the proliferation of C6 glioma stem cells, which was mediated through enhanced Wnt/β‑catenin signaling, which is associated with glioma tumor aggressiveness. LDR treatment increased the expression of the DNA damage marker γ‑H2AX but promoted cell survival with a significant reduction in apoptotic and necrotic cells. When LDR cells were also treated with an inhibitor of Wnt receptor 1 (IWR1), cell proliferation and migration were significantly reduced. IWR1 treatment significantly inhibited Wnt1, Wnt3a and β‑catenin protein expression. Collectively, the current results demonstrated that IWR1 treatment effectively radio‑sensitizes glioma stem cells and helps to overcome the survival advantages promoted by LDR, which has significant implications for targeted treatment in radioresistant gliomas.
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February-2024
Volume 51 Issue 2

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Spandidos Publications style
Yan Y, Cheng Y, Li Y, Jiao X, Liu Y, Cai H and Ding Y: Inhibitor of Wnt receptor 1 suppresses the effects of Wnt1, Wnt3a and β‑catenin on the proliferation and migration of C6 GSCs induced by low‑dose radiation. Oncol Rep 51: 22, 2024.
APA
Yan, Y., Cheng, Y., Li, Y., Jiao, X., Liu, Y., Cai, H., & Ding, Y. (2024). Inhibitor of Wnt receptor 1 suppresses the effects of Wnt1, Wnt3a and β‑catenin on the proliferation and migration of C6 GSCs induced by low‑dose radiation. Oncology Reports, 51, 22. https://doi.org/10.3892/or.2023.8681
MLA
Yan, Y., Cheng, Y., Li, Y., Jiao, X., Liu, Y., Cai, H., Ding, Y."Inhibitor of Wnt receptor 1 suppresses the effects of Wnt1, Wnt3a and β‑catenin on the proliferation and migration of C6 GSCs induced by low‑dose radiation". Oncology Reports 51.2 (2024): 22.
Chicago
Yan, Y., Cheng, Y., Li, Y., Jiao, X., Liu, Y., Cai, H., Ding, Y."Inhibitor of Wnt receptor 1 suppresses the effects of Wnt1, Wnt3a and β‑catenin on the proliferation and migration of C6 GSCs induced by low‑dose radiation". Oncology Reports 51, no. 2 (2024): 22. https://doi.org/10.3892/or.2023.8681